Audition Sisters focuses on five women with distinct personalities. Orphan Chen Man mistook Zhang Yuening due to a birthmark, and after going blind, was betrayed by her and Su Qingran who lusted after her family estate, fortunately being protected by Lin Xiaoyu and Xu Jiaqi. Through legal sanction and psychological counseling, Zhang Yuening and Su Qingran sincerely repented, and the five set aside their grievances to join hands in auditioning and chasing their dreams. Enduring multiple hardships such as cross-dressing blunders, costume controversies, provocations, and family misunderstandings, they always held firm to the original aspiration of "relying on strength to make drama", supporting each other and achieving growth through practicing acting and facing challenges. In the end, Chen Man won the female lead role unanimously with superb acting skills, and everyone, tested by auditions and life, lived to become each other's support, interpreting the power of women helping women.